Animal-Free Blocking Solution (5X) from Cell Signaling Technology

Product Specs
ItemAnimal-Free Blocking Solution (5X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number15019L
Quantity250 ml
ApplicationsW, IHC-P
Research AreaRelated Products

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Characterization and optimization of variability in a human colonic epithelium culture modelbioRxiv : the preprint server for biologySeptember 22, 2023Pike CM, Zwarycz B, McQueen BE, Castillo M, Barron C, Morowitz JM, Levi JA, Phadke D, Balik-Meisner M, Mav D, et al.antibodies were added at 1:250 dilution in 1X Animal-Free Blocking Solution (Cell Signaling Technology, 15019L) to the apical side of each Transwell® for an overnight incubation at 4°C. Secondary antibodies and

(2) Total Flavonoids of Rhizoma Drynariae Treat Osteoarthritis by Inhibiting Arachidonic Acid Metabolites Through AMPK/NFκB PathwayJournal of Inflammation ResearchSeptember 20, 2023Guang-Yao Chen, Xiao-Yu Liu, Xue-Er Yan, XinBo Yu, Yi Liu, Jing Luo, Qing-Wen TaoHyClone, USA), RIPA lysis buffer (R0010, Solarbio Life Sciences, China), Animal free blocking solution (15019S, Cell Signaling Technology, USA), Protease and phosphatase inhibitor cocktail mix (P1261, Solarbio Life

(3) The spatiotemporal dynamics of spatially variable genes in developing mouse brain revealed by a novel computational schemeCell Death DiscoveryJuly 27, 2023Yingzhou Hong, Kai Song, Zongbo Zhang, Yuxia Deng, Xue Zhang, Jinqian Zhao, Jun Jiang, Qing Zhang, Chunming Guo, Cheng Pengincubated in H2O2 for 10 min for bleaching non-specific HRP reaction. Animal-free blocking solution (CST, 15019) was used for the blocking, then primary antibodies (Supplementary Table 6) were mixed with 50 times

(4) Activating an Adaptive Immune Response with a Telomerase-Mediated Telomere Targeting Therapeutic in Hepatocellular CarcinomaMolecular cancer therapeuticsJune 1, 2023Mender I, Siteni S, Barron S, Flusche AM, Kubota N, Yu C, Cornelius C, Tedone E, Maziveyi M, Grichuk A, et al.biotin were blocked using 1× Animal-Free Blocking Solution (Cell Signaling Technology, Catalog No. 15019). Sections were then washed twice with PBS for 5 minutes each. Primary antibodies were applied overnight

(5) Cell death-induced immunogenicity enhances chemoimmunotherapeutic response by converting immune-excluded into T-cell inflamed bladder tumorsNature CommunicationsDecember 1, 2022Fotis Nikolos et al.steamer. Tissue sections were blocked with animal-free blocking solution (Cell Signaling Technologies, 15019) for 10 min and incubated with primary antibodies against CD3 (Abcam, ab5690, 1:200), COX-2 (Cell Signaling Technologies

(6) Cutaneous burn injury induces neuroinflammation and reactive astrocyte activation in the hippocampus of aged miceExperimental GerontologyNovember 1, 2022Travis Walrath, Rachel H. McMahan, Juan-Pablo Idrovo, Nidia Quillinan, Elizabeth J. KovacsVector), quenching of endogenous peroxidase activity, and blocked with animal-free blocking solution (cat#15019L, Cell Signaling). pCREB was visualized by incubation with 1:500 dilution of primary antibody (#ab32096,

(7) DUSP4 protects BRAF- and NRAS-mutant melanoma from oncogene overdose through modulation of MITFLife Science AllianceSeptember 1, 2022Nuria Gutierrez-Prat et al.Nitrocellulose membrane (#1704159; Bio-Rad). Then, membranes were blocked using 1× of Animal-Free Blocking Solution (#15019; Cell Signaling) in TBS 0.01% Tween for 1 h at RT. After blocking, membranes were incubated at 4°C overnight

(8) Effect of the Combination of Everolimus and Mesenchymal Stromal Cells on Regulatory T Cells Levels and in a Liver Transplant Rejection Model in RatsFrontiers in ImmunologyJune 10, 2022Morgan Vandermeulen, Pauline Erpicum, Noella Bletard, Laurence Poma, François Jouret, Olivier Detrybinding was constrained by incubation for 10min with protein block reagent (Cell Signaling Technology, #15019L). Then, sections were incubated for 60min at room temperature with primary antibodies: Rabbit anti FoxP3

(9) Network Pharmacology Analysis and Experimental Validation to Investigate the Mechanism of Total Flavonoids of Rhizoma Drynariae in Treating Rheumatoid ArthritisDrug design, development and therapyJanuary 1, 2022Chen GY, Luo J, Liu Y, Yu XB, Liu XY, Tao QW.Polyvinylidene difluoride (PVDF) membranes (ISEQ00010, Millipore, USA), Animal-free Blocking Solution (15019S, Cell Signaling, USA), RIPA lysis buffer (R0010, Solarbio, China), Phenylmethanesulfonyl fluoride (PMSF)

(10) Mechanical Stimulation Protects Against Chondrocyte Pyroptosis Through Irisin-Induced Suppression of PI3K/Akt/NF-κB Signal Pathway in OsteoarthritisFrontiers in cell and developmental biologyJanuary 1, 2022Jia S, Yang Y, Bai Y, Wei Y, Zhang H, Tian Y, Liu J, Bai L.H2O2 was used at 25°C for 30 min to eliminate endogenous peroxidase activity, while blocking serum (15019, Cell Signaling Technology, Danvers, MA, United States) was used at 25°C for 30 min to block nonspecific

(11) Dissociation of disease onset, progression and sex differences from androgen receptor levels in a mouse model of amyotrophic lateral sclerosisScientific ReportsDecember 1, 2021Doris Tomas, Victoria M. McLeod, Mathew D. F. Chiam, Nayomi Wanniarachchillage, Wah C. Boon, Bradley J. Turnerin PBS and 1 h at room temperature in animal-free blocking reagent (Cell Signalling Technology, Cat# 15019). Rabbit anti-AR (as described above) was incubated at 4 °C for 48 h in SignalStain antibody diluent

(12) Tumor resistance to ferroptosis driven by Stearoyl-CoA Desaturase-1 (SCD1) in cancer cells and Fatty Acid Biding Protein-4 (FABP4) in tumor microenvironment promote tumor recurrenceRedox biologyJuly 1, 2021Luis G, Godfroid A, Nishiumi S, Cimino J, Blacher S, Maquoi E, Wery C, Collignon A, Longuespée R, Montero-Ruiz L, et al.For the detection of PLIN2, slides were incubated with animal free blocking solution (Cell Signaling, 15019L) for 20 min at room temperature (RT). For SCD1 and pimonidazole staining, non-specific sites were blocked

(13) Dual therapeutic targeting of intra-articular inflammation and intracellular bacteria enhances chondroprotection in septic arthritisScience advancesJune 1, 2021Kwon HK, Lee I, Yu KE, Cahill SV, Alder KD, Lee S, Dussik CM, Back J, Choi J, Song L, et al.were then incubated with Animal-Free Blocking Solution (Cell Signaling Technology Inc., catalog no. #15019) for 30 min and incubated with the desired primary antibody at a 1:200 dilution for 60 min. After primary

(14) Arrhythmogenic Hearts in PKD2 Mutant Mice Are Characterized by Cardiac Fibrosis, Systolic, and Diastolic DysfunctionsFrontiers in cardiovascular medicineJanuary 1, 2021Amirrad F, Pala R, Shamloo K, Muntean BS, Nauli SM.were incubated in 3% hydrogen peroxide for 10 min and blocked with animal-free blocking solution (Cat# 15019; Cell Signaling, Inc.). After blocking, slides were incubated overnight at 4°C with mouse monoclonal

(15) Sox9^EGFP Defines Biliary Epithelial Heterogeneity Downstream of Yap ActivityCellular and molecular gastroenterology and hepatologyJanuary 1, 2021Tulasi DY, Castaneda DM, Wager K, Hogan CB, Alcedo KP, Raab JR, Gracz AD.minutes and then blocked in 1X Animal-Free Blocking Solution (Cell Signaling Technology, Danvers, MA; 15019) for 30 minutes. Primary antibodies were applied in PBS for 2 hours at room temperature or overnight

(16) Integrative discovery of treatments for high-risk neuroblastomaNature CommunicationsDecember 1, 2020Elin Almstedt et al.Antigen Retriever (Aptum Biologics Ltd.), sections were blocked with Animal-free blocking solution (#15019, Cell Signaling Technology), primary antibodies were diluted in normal antibody diluent (Immunologic),

(17) ARID1A Mutations Promote P300-Dependent Endometrial Invasion through Super-Enhancer HyperacetylationCell reportsNovember 10, 2020Wilson MR, Reske JJ, Holladay J, Neupane S, Ngo J, Cuthrell N, Wegener M, Rhodes M, Adams M, Sheridan R, et al.,Cell Signaling Technology,Cat# 8112L Animal-Free Blocking Solution (5X),Cell Signaling Technology,Cat# 15019L "VECTASTAIN Elite ABC-HRP Kit (Peroxidase, Standard)",Vector Laboratories,Cat# PK-6100 ImmPACT DAB

(18) Apigenin induces apoptosis by regulating Akt and MAPK pathways in human melanoma cell A375SMMolecular Medicine ReportsOctober 8, 2020Joong‑Seok Woo et al.Sections were blocked using 1X Animal-Free Blocking Solution (Cell Signaling Technology, Inc., cat. no. 15019) at room temperature for 1 h. The sections were incubated with an antibody against p-ERK (1:100) at

(19) Reduced Dendritic Spines in the Visual Cortex Contralateral to the Optic Nerve Crush Eye in Adult MiceInvestigative Opthalmology & Visual ScienceSeptember 1, 2020Zongyi Zhan, Yali Wu, Zitian Liu, Yadan Quan, Deling Li, Yiru Huang, Shana Yang, Kaili Wu, Lianyan Huang, Minbin Yuand washed twice using distilled water. Each section was blocked with animal-free blocking solution (15019; Cell Signaling Technology, Boston, MA, USA) for 1 hour at room temperature (RT) and then incubated

(20) Removal of N-Linked Glycosylation Enhances PD-L1 Detection and Predicts Anti-PD-1/PD-L1 Therapeutic EfficacyCancer cellAugust 12, 2019Lee HH, Wang YN, Xia W, Chen CH, Rau KM, Ye L, Wei Y, Chou CK, Wang SC, Yan M, et al.ELISA,Santa Cruz Biotechnology,Cat#293965 Animal-Free Blocking Solution,Cell Signaling Technology,Cat#15019 "Concanavalin A, HRP-conjugated",Sigma-Aldrich,Cat#L6397 ,, Critical Commercial Assays,Critical Commercial

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Animal-Free Blocking Solution (5X) from Cell Signaling Technology

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Animal-Free Blocking Solution (5X) from Cell Signaling Technology

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